Journal: The Journal of Neuroscience

Article Title: Postsynaptic Expression of a New Calcium Pathway in Hippocampal CA3 Neurons and Its Influence on Mossy Fiber Long-Term Potentiation

doi: 10.1523/JNEUROSCI.22-11-04312.2002

Figure Lengend Snippet: Sindbis viral-mediated expression of GluR2Q in hippocampal neurons in monolayer culture. Dissociated neurons derived from the area including the CA3 region and the dentate gyrus of the rat hippocampus were infected with SIN-myc-EG-GluR2Q for expression of the myc-GFP-tagged GluR2Q fusion protein 18 hr before examination.A, Infected neurons emitting GFP fluorescence.B, Cell-surface expression of myc-GFP-tagged GluR2Q fusion protein. Cell-surface staining was performed using anti-myc antibody and Alexa594-conjugated secondary antibody (red). C, A merged image ofA and B. D, Colocalization of myc-tagged GluR2Q (red) and synaptophysin (purple) immunoreactivity. After staining for cell-surface expression of myc-GFP-tagged GluR2Q, cells were permeabilized and then treated with anti-synaptophysin antibody and Cy5-conjugated secondary antibody. Arrowheads indicate colocalization. Scale bars: A–C, 20 μm;D, 5 μm.

Article Snippet: Subsequently, to confirm the exogenous GluR2Q expression on the postsynaptic membranes, samples were reacted with polyclonal anti-synaptophysin antibody (1:100; PharMingen, San Diego, CA) for 1 hr at room temperature after permeabilization with 0.1% Triton X-100 and subsequent incubation in 10% normal goat serum in PBS to block nonspecific binding.

Techniques: Expressing, Derivative Assay, Infection, Fluorescence, Staining

Journal: International Journal of Molecular Sciences

Article Title: Cerebral Benefits Induced by Electrical Muscle Stimulation: Evidence from a Human and Rat Study

doi: 10.3390/ijms25031883

Figure Lengend Snippet: Primary antibodies.

Article Snippet: SYP , Interchim rabbit polyclonal RB-1461-P1 , 1/3000 TBST-Milk (5%).

Techniques: Recombinant, Transduction, Purification